AlgaBoost EPA-1002/1003 no EDTA is a 400x concentrate of the medium used for Toxicity Testing. It is identical to AlgaBoost EPA-1002/1003, except for the absence of EDTA.

Application : medium of choice for heavy metal toxicity tests; the exclusion of EDTA from this formulation allows more sensitive detection of heavy metals.

Makes: When diluted in water according to instructions, it makes the following media:

        1. EPA Test Method 1002.0 (but EDTA free) (Daphnid Ceriodaphnia dubia, Survival and Reproduction Test).

        2. EPA Test Method 1003.0 (but EDTA free) (Green Alga, Selenastrum capricornutum, Growth Test).

To Make:     
    2 litres distilled water
+    1 x 5ml Syringe Part A
+    1 x 5ml Syringe Part B
    2 litres USEPA-1002/1003 medium (EDTA-free)

Nutrient Balance: When added to your seawater + freshwater mix (seawater : freshwater = 4 : 1.1 ) according to the accompanying
instructions, the combined parts of this formulation will provide the following nutrient levels:

Nutrient	Final conc per litre (g/l)
NaNO3	25.5 mg
NaHCO3	15 mg
MgSO4.7H2O	14.7 mg
MgCl2.6H2O	12.2 mg
CaCl2.2H2O	4.41mg
K2HPO4	1.04 mg
MnCl2.4H2O	0.416 mg
H3BO3	0.185 mg
FeCl3.6H2O	0.160 mg
CoCl2.6H2O	0.00143 mg
ZnCl2	0.00327 mg
Na2SeO4	0.00239 mg
CuCl2.2H2O	0.000012 mg
Na2MoO4.2H20	0.00726 mg

Nutrient Balance: When added to freshwater according to the accompanying instructions, this formulation will provide the following nutrient levels:
           
How is the product supplied ?   - As a 2-part mix, in packs containing 12 kits (enough to make 12 x 2 litres of EPA 1002/1003 medium).   Each part of the 2-part mix is sterile and is provided in a convenient 5ml syringe; the two syringes are provided in a sealed sterile pack.

To Make the Medium:    - Open a 2-syringe kit.  Then add the the contents of one 5ml syringe to 2 litres of pure water, mix thoroughly, then add contents of the other 5 ml syringe and mix (instructions accompany the product).  Aerate the mixture, adjust pH if necessary (recommended for EPA method 1003.0) and filter sterilise.

NOTE: EPA-1002/1003 is sterile; the final sterilisation step (described above) is necessary only if diluent (water) is not sterile and/or if sterile technique has not been used while preparing the final medium.

Reference (EDTA-version): "Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms." Eds: P.A. Lewis, D.J. Klenni, J.M Lazorchak, T.J. Norberg-King, W.H. Peltier, M.A. Heber 1994.

Availability:  'EPA-1002/1003 no EDTA' is presently available only in the syringes. If you are interested in purchasing larger amounts, please enquire with us.